IITAA

O Instituto de Investigação e Tecnologia da Agronomia e Meio Ambiente (IITAA) desenvolve trabalho de investigação com o objetivo de perseguir estudos em diversas áreas, como a caracterização / previsão do clima insular e os efeitos das mudanças globais em comunidades do oceano para os to... Ler mais »

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In Vitro Effect of the Reproductive Hormones on the Oxidative Burst Activity of Polymorphonuclear Leucocytes from Cows: A Flow Cytometric Study.

Agricultura e Produção Animal

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Referência

A. Chaveiro, F. Moreira da Silva. 2009. In vitro effect of the reproductive hormones on the oxidative burst activity of polymorphonuclear leukocytes from cows: a flow cytometric study. Reproduction in Domestic Animals. (Epub ahed of print)

Resumo

Content In this study, the effect of reproductive hormones and substances with hormonal activity on the oxidative burst activity of blood polymorphonuclear leucocytes (PMN) high yielding dairy cows was evaluated. Different concentrations of: progesterone, oestradiol 17beta, FSH, LH, GnRH, cortisol and PGF2alpha were incubated in vitro for 4 h with PMN of seven high milk yielding cows, during the period of anoestrous postpartum. Controls were run in parallel in which each hormone was replaced by its solvent. After incubation with hormones the competence of PMN to generate H(2)O(2) was monitored by flow cytometry. A down-regulation on the oxidative burst activity of PMA-stimulated PMN was observed when cells were incubated with progesterone. Significant (p

Segunda, 16 Novembro, 2009

Equipa

Joaquim Fernando Moreira da Silva
Membro Integrado com contrato
António Eduardo Nobre Chaveiro
Investigador Colaborador (Antigo membro integrado)

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