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O Instituto de Investigação e Tecnologia da Agronomia e Meio Ambiente (IITAA) desenvolve trabalho de investigação com o objetivo de perseguir estudos em diversas áreas, como a caracterização / previsão do clima insular e os efeitos das mudanças globais em comunidades do oceano para os to... Ler mais »

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Effect of α-tocopherol on in vitro culture of bovine embryos

Agricultura e Produção Animal

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Referência

A. Marques, G. Antunes, P. Santos, A. Chaveiro e F. Moreira da Silva. 2007. Effect of α-tocopherol on in vitro production of bovine embryos. Canadian Journal of Animal Science. 87: 539–542

Resumo

Bovine oocytes were matured and fertilised under 5% CO2. Presumptive zygotes were co-cultured in synthetic oviductal fluid droplets, supplemented with either 0 (control), 25, 50, 100, or 200 µM of α-tocopherol. Blastocyst development rates were significantly influenced (P < 0.05) by the level of antioxidant in the culture media. Control showed a blastocyst yield of 18.46, 21.11, 27.92, and 31.66%, respectively, at α25; α50 and α100. Blastocyst yield for α200 was severely decreased, to 8.01%. An increase in overall IVF results was also observed as the concentration of α-tocopherol increased (control, 14.21%; α25, 14.35%; α50, 19.52%; α100, 21.11%), decreasing to 5.75% for the concentration of α200. The present study clearly demonstrates that α-tocopherol at a concentration of 100 µM significantly improves the proportion of oocytes that develop to the blastocyst stage.

Sbado, 01 Dezembro, 2007

Equipa

Joaquim Fernando Moreira da Silva
Membro Integrado com contrato
António Eduardo Nobre Chaveiro
Investigador Colaborador (Antigo membro integrado)

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