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Effect of α-tocopherol on in vitro maturation of bovine cumulus-oocyte complexes
Agricultural and Animal Production
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Reference
Marques, A., Antunes, G., Santos, P., Chaveiro, A. e Moreira da Silva, F. 2008. Effect of alpha tocopherol on in vitro maturation of bovine cumulus oocyte complexes. Canadian Journal of Animal Science. 88(3):463-467
Summary
This study determined: the effects of α-tocopherol on apoptotic and necrotic levels of cumulus cells after in vitro maturation of bovine oocytes; whether exposure to α-tocopherol facilitates the development of bovine enclosed oocytes to metaphase II; and the effects of this antioxidant on apoptotic and necrotic levels of granulosa cells cultured in vitro. In conclusion, supplementation with α-tocopherol on in vitro maturation of bovine oocytes has a detrimental effect on the ability of oocytes to reach metaphase II, increasing the number of apoptotic and necrotic cumulus cells of bovine cumulus oocyte complexes (COC). This antioxidant showed a slight improvement in the viability of cultured granulosa cells at a concentration of 100 µM. Key words: Bovine, oocyte maturation in vitro, antioxidant, α-tocopherol
Monday, 01 September, 2008
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