IITAA

O Instituto de Investigação e Tecnologia da Agronomia e Meio Ambiente (IITAA) desenvolve trabalho de investigação com o objetivo de perseguir estudos em diversas áreas, como a caracterização / previsão do clima insular e os efeitos das mudanças globais em comunidades do oceano para os to... Ler mais »

PUBLICAÇÕES

Assessment of Sperm Apoptosis in Cryopreserved Bull Semen After Swim-up Treatment: A Flow Cytometric Study

Agricultura e Produção Animal

+ Info

Referência

Chaveiro A, Santos P, Moreira da Silva F. (2007). Assessment of sperm apoptosis in cryopreserved bull semen after swim-up treatment: A flow cytometric study. Reproduction in Domestic Animals, 42(1), 17–21.

Resumo

The techniques used to prepare bovine spermatozoa for in vitro fertilization, to enhance the percentage of motile sperm cells include the swim-up (SU) method, among others. The objective of the present study was to evaluate the phosphatidylserine (PS) translocation and plasma membrane integrity as the indicator of apoptosis and necrosis in post-thaw bull sperm after SU treatment using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) assay. A flow cytometric method was employed to measure apoptosis levels on frozen-thawed bull spermatozoa. The assay detects PS translocation across the plasma membrane using a fluorescein-labelled annexin-V and PI. By using the annexin V/PI assay four different subpopulations of sperm were observed: (i) a population of apoptotic sperm, labelled with annexin V–FITC but not with PI; (ii) a population of early necrotic spermatozoa, sperm labelled with annexin–FITC and PI; (iii) a population of necrotic sperm, labelled with PI but not with annexin–FITC; and (iv) a population of fully viable sperm cells, sperm not labelled with annexin V–FITC and without PI. Results clearly indicated that SU technique itself could have an adverse effect on the spermatozoa membrane stability. It has also been found, significant differences between bulls in the levels of apoptotic sperm, after SU treatment.

Quinta, 01 Fevereiro, 2007

Equipa

Joaquim Fernando Moreira da Silva
Membro Integrado com contrato
António Eduardo Nobre Chaveiro
Investigador Colaborador (Antigo membro integrado)

    Ver todas as publicações

 
Web Development: Via Oceânica, 2009
English