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Preliminary screening of sulfur vents for antibiotic-producing microorganisms

Tecnologias Alimentares e Saúde

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Referência

Riquelme C., Varela A.R., Ghadimi A.M., Moselhy M.A., Hathaway J.M.M.,
Dapkevicius M.L.N.E., Northup D.E. 2010 “Preliminary screening
of sulfur vents for antibiotic-producing microorganisms” 8th International Congress on Extremophiles. São Miguel, Azores

Resumo

Sulfur vents like the ones found in the island of Terceira, Azores (North Atlantic;37–40°N, 25–31°W), are home to extremophilic microorganisms adapted to survive in harsh ecological niches with stressing conditions in terms of temperature, pH and sulfur concentrations. It has been suggested and demonstrated that adaptation responses of microorganisms under stress may include the production of metabolites or enzymes useful to humans (1,2). In this work, we collected a small sample from microorganisms living in soil around sulfur vents and tested their activity against food-borne human pathogens (Proteus sp., Salmonella sp., Escherichia coli ATCC 25922, Listeria monocytogenes, Staphylococcus aureus 3DA, Staphylococcus aureus ATCC 9144, Pseudomonas aeruginosa) and a non-pathogenicListeria innocua. Isolates displaying a large-spectrum of inhibition against the target microorganisms were sequenced and identified. DNA was extracted from the cultured samples, and the 16S rRNA gene was sequenced using universal bacterial primers.  The resulting sequences were compared to known sequences from GenBank, and phylogenetic trees were constructed to determine relationships among these cultures. Sulfur vents are an under explored source of potential antibiotic producing microorganisms.

Quinta, 16 Setembro, 2010

Equipa

Maria de Lurdes Nunes Enes Dapkevicius
Membro Integrado com contrato
Cristina Riquelme Gabriel
Investigador Colaborador (Outros)
Ana Rita Boura Varela
Antigo membro

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